Real-Time PCR Genotyping

GTCA uses high-throughput, real-time PCR genotyping assays for common transgene targets, such as neomycin, luciferase, GFP, lacZ, and Cre recombinase, as well as custom gene-specific assay development for targeted mutations (knock-ins/knock-outs). We also use probe-based PCR for SNP (Single Nucleotide Polymorphism) and Indel (insertion and deletion) testing for large- and small-scale projects including SNP, validation, and screening.

DNA is extracted using our proprietary methods and your samples are tested in duplicate to ensure accuracy. Results are delivered within 2 business days, once assays are designed.

For targeted mutations, we determine and report on zygosity for each sample. Transgenic samples have presence/absence reported (designated as Negative or Positive – homozygous are not differentiated.) The zygosity of transgenic samples is determined with our higher-level transgene zygosity testing service.