Assay Design

We pride ourselves on developing the best assays and using the most appropriate methods for your genotyping needs. The nature and structure of the mutation present in your strain, we will inform the strategy for genotyping your strain. Below is a list of information that is needed to initiate assay designs. Once you have this information you can request a new assay here.

Information needed to initiate a new assay design:

Strain/line name – both your in-house name and official strain nomenclature, if different.
Name of vendor and stock #, if it is a commercially available strain
Map and/or description of how the mutant was produced, and its location.
If it is a transgene, do anticipate producing homozygotes (i.e., do you need to know zygosity)?
Known positive controls. Ideally, homozygous, heterozygous or hemizygous. See our FAQ (“What terminology is used to describe results?”) for an explanation of zygosity nomenclature.

If available, please also provide the following:

Complete sequence
Current genotyping method and primer sequences

If you do not have all of the needed information, please contact us. We may be able to find missing information from commercial vendor websites or other resources.

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